ice inference of crispr edits software (Synthego Inc)
Structured Review

Ice Inference Of Crispr Edits Software, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/inference+crispr+edits+(ice/ice+synthego/bio_rxiv__64898__2026__05__13__724745-111-3-10
Average 86 stars, based on 1 article reviews
Images
1) Product Images from "CRISPR/Cas12a-Mediated Knockout of the INNER NO OUTER ( INO ) Gene in Musa balbisiana cv. Bhimkol"
Article Title: CRISPR/Cas12a-Mediated Knockout of the INNER NO OUTER ( INO ) Gene in Musa balbisiana cv. Bhimkol
Journal: bioRxiv
doi: 10.64898/2026.05.13.724745
Figure Legend Snippet: A) Induction of embryonic calli from male flowers of Bhimkol after 6 months of culture. B) A magnified view at 40x of embryogenic calli. C) Establishment of ECS after 8 months of culture was used for genetic transformation mediated through Agrobacterium harboring pSS03. D) Embryonic calli in selection medium with 40 mg/L hygromycin, and 250 mg/L timentin. E) A magnified view of putative transformed mature somatic embryos at 40x magnification. F) Shoots regeneration in M4 medium containing 40 mg/L hygromycin, and 250 mg/L timentin after one month of culture. G) Rooting from i n vitro regenerated shoots in the R1 rooting medium containing 40 mg/L hygromycin, and 250 mg/L timentin after 14 days of culture. H) Putative CRISPR-edited Bhimkol plantlets with healthy shoots and roots, ready for hardening.
Techniques Used: Transformation Assay, Selection, CRISPR
Figure Legend Snippet: CRISPR-edited Bhimkol plants transferred to the transgenic net house, A) 03_01, B) 03_05, C) 03_06, D) 03_17 and E) Wild Type (Control)
Techniques Used: CRISPR, Transgenic Assay, Control
Figure Legend Snippet: Sequence analysis of the INO gene in CRISPR/Cas12a-edited lines. Amino acid sequences from lines 03_01, 03_05, 03_06, and 03_17 were aligned with the wild type sequence. Red asterisks indicate stop codons.
Techniques Used: Sequencing, CRISPR
Figure Legend Snippet: Analysis of YABBY and HMG-box_2 domain in the WT and CRISPR-edited lines. The CRISPR-edited Bhimkol lines exhibit a complete absence of the HMG_box_2 and truncated YABBY domains.
Techniques Used: CRISPR
Related Articles
CRISPR:Article Title: Dual inhibition of intercellular adhesion molecule-1 and nucleolin reduces RSV infection efficiency in human respiratory organoids Article Snippet: .. Analysis was done using inference of Article Title: Aging restricts maturation of CXCL13 + T follicular helper cells in human immunity. Article Snippet: Successful PCR amplification was confirmed by agarose gel electrophoresis and PCR amplicons were transferred to ELIM Biopharm for Sanger sequencing. .. Editing efficiencies (Table S3) were calculated using the Inference of Article Title: CRISPR/Cas12a-Mediated Knockout of the INNER NO OUTER ( INO ) Gene in Musa balbisiana cv. Bhimkol Article Snippet: .. Utilizing Article Title: CRISPR/Cas12a-Mediated Knockout of the INNER NO OUTER ( INO ) Gene in Musa balbisiana cv. Bhimkol Article Snippet: .. Furthermore, analysis using Article Title: High genotoxicity of CRISPR/Cas9 versus limited efficacy of CRISPRi in chicken primordial germ cells Article Snippet: .. The amplicons were subjected to Sanger sequencing, and sequencing traces were analyzed using the Inference of Article Title: A mitochondria-driven quality control mechanism for peroxisomal membrane proteins. Article Snippet: .. Editing efficiency was assessed using the Inference of Gene Knockout:Article Title: Aging restricts maturation of CXCL13 + T follicular helper cells in human immunity. Article Snippet: Successful PCR amplification was confirmed by agarose gel electrophoresis and PCR amplicons were transferred to ELIM Biopharm for Sanger sequencing. .. Editing efficiencies (Table S3) were calculated using the Inference of Software:Article Title: CRISPR/Cas12a-Mediated Knockout of the INNER NO OUTER ( INO ) Gene in Musa balbisiana cv. Bhimkol Article Snippet: .. Utilizing Article Title: CRISPR/Cas12a-Mediated Knockout of the INNER NO OUTER ( INO ) Gene in Musa balbisiana cv. Bhimkol Article Snippet: .. Furthermore, analysis using Article Title: High genotoxicity of CRISPR/Cas9 versus limited efficacy of CRISPRi in chicken primordial germ cells Article Snippet: .. The amplicons were subjected to Sanger sequencing, and sequencing traces were analyzed using the Inference of Sequencing:Article Title: High genotoxicity of CRISPR/Cas9 versus limited efficacy of CRISPRi in chicken primordial germ cells Article Snippet: .. The amplicons were subjected to Sanger sequencing, and sequencing traces were analyzed using the Inference of other:Article Title: Optimized lentivirus-derived virus-like particles for efficient delivery of Cas9-based genome editors. Article Snippet: Cells were collected and washed with 1 ml phosphate buffered saline (PBS), then stained with B2M-PE (clone 2M2, BioLegend, San Diego, CA), CD45-APC (clone HI30, BioLegend), CD44-PE-Cy7(clone IM7, BioLegend), CD29-APC (clone MAR4, BD Biosciences, WUborn, MA), CD47-FITC (clone miap301, BioLegend), and viability dye (Fixable Viability Dye eFluor 780, eBioscience San Diego, California) antibodies at 4◦C for 20 min at dilutions recommended by the manufacturer. |

